treatment of neurological disorders

CERTIFICATE

This is to certify that Dr. Syed Ameer Basha Paspala has carried out the research work presented in the thesis entitled "Isolation and identification of neural stem cells from human fetal brain for the treatment of neurological disorders" This work has been carried out under my guidance and supervision at Centre for Liver Research and Diagnostics, Deccan college of Medical Sciences and allied hospital, Kanchanbagh, Hyderabad. I hereby recommend the submission of this thesis for the degree of Doctor of Philosophy in Biotechnology.

Hyderabad Dr M N Khaja

Date : Supervisor

CERTIFICATE

This is to certify that DR. Syed Ameer Basha Paspala has carried out the research work presented in the thesis entitled "Isolation and identification of neural stem cells from human fetal brain for the treatment of neurological disorders". This work has been carried out under my guidance and supervision at Centre for Liver Research and Diagnostics, Deccan college of Medical Sciences and allied hospital, Kanchanbagh, Hyderabad. I hereby recommend the submission of this thesis for the degree of Doctor of Philosophy in Biotechnology.

Hyderabad Dr. Y. Prameela Devi

Date : Co-Supervisor

DECLARATION

I hereby declare that the work incorporated in this thesis entitled "Isolation and identification of neural stem cells from human fetal brain for the treatment of neurological disorders" has been carried out by me under the supervision of Dr. M. N. Khaja and Co-supervision of Dr. Y. Prameela Devi at Centre for Liver Research and Diagnostics, Deccan college of Medical Sciences and allied hospital, Kanchanbagh and Dept of Biotechnology, JNTU, Hyderabad.

This thesis is the presentation of original research work carried out by me and it has not been submitted, in part or in full, for the award of any other degree or diploma of any university.

Hyderabad Dr. Syed Ameer Basha Paspala

Date :

ACKNOWLEDGEMENT

I take this opportunity to express my deepest sense of gratitude and sincere thanks to my guide Dr. MN Khaja, Scientist, Centre for Liver research and Diagnostics, Deccan College of Medical Sciences, Hyderabad, for his valuable guidance, suggestions, encouragement and constant support throughout the tenure of this work .

I am extremely thankful to my Co-guide Dr.Y. Pramila Devi, JNTU, Hyderabad. I express my sincere thanks to Dr. Lakshmi Narasu, Director of IST, JNTU, Hyderabad for their constant encouragement and support right from the initiation to the completion of this study.

I am highly indebted to Dr. C. M. Habibullah, Director, Deccan College of Medical Sciences & CLRD, Hyderabad for his support, unabiding guidance and constant encouragement. It is primarily through him that I have been exposed to the fascinating subject of Stem Cell Research and Molecular Biology. He has been a source of inspiration and encouragement to me all along.

I express my sincere thanks to Dr. Aleem Ahmed Khan, Scientist, Centre for Liver Research & Diagnostics (CLRD) for his ardent encouragement, valuable suggestions and guidance throughout the study.

I wish to express my thanks to Dr. Parveen, Scientist, CLRD for her support and constant encouragement throughout the study.

I express my sincere thanks to Mr.A.B.Balaji, who has supported me with their constant encouragement and valuable suggestions.

I am grateful to Dr. V. V. Ramachandra Rao, Scientist for the help and valuable guidance extended to me during my Pre-PhD. exam preparations.

I am thankful to Dr. Lalji Singh, Director, Centre for Cellular and Molecular Biology (CCMB) for the facilities provided and Dr. Gopal Pande, Scientist, CCMB for his co-operation during my work in CCMB.

I extend my thanks to Dr. Zakia Abid, Head, Dept. of Pathology, Deccan College of Medical Sciences, Hyderabad for the facilities provided to carry out my work.

I would like to thank the entire team of Centre for Liver Research & Diagnostics, Hyderabad, especially, Dr. Madhavi, Mr. Santhosh Tiwari, Mr. Shakeel, Mr. Manoj, Mr. Mahboob Vali Shaik & Mr.Rajendraprasad for their timely help and kind co-operation given to me during the entire course of my study.

It gives me immense pleasure in expressing my sincere thanks to my wife Dr. Athiya and my children for having endowed me not only with their affection and constant encouragement, but also for having stood by me through thick and thin and without their support, the thesis could not have been completed.

The moral support & encouragement received from my brother-in-law Dr. Md Aejaz Habeeb , my nieces & nephew is highly acknowledged.

I would like to thank the entire staff of CLRD especially Mr. Nasser Akbar, Mr. J. V. Chalapathi and Ms. Athiya for their support.

Dedicated to

My Beloved Parents

And

My Wife

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