Dengue virus

PanBio Dengue Duo IgM and IgG Rapid Cassette

- 30-45 min

- Both IgM and IgG in single well, save cost

- Efficiency 3

- Not good in detecting IgG

PanBio Dengue Duo IgM and IgG Capture ELISA

- 2-4 hrs

- Both IgM and IgG in single well, save cost

- Efficiency 2

Accusens Dengue Virus Rapid Strip Test

- 30-45 min

- Efficiency 4

Unitest Dengue IgM and IgG Combo Rapid Test

- 30-45 min

- Efficiency 5

Anti-Dengue Virus IgM Detection PA Kit 96 (Pentax Corporation)

- 2-4 hrs

- Efficiency 1

Name : Sum Jia Siang

If you were establishing a serological diagnostic service for dengue diagnosis, how would these data affect the way in which you introduce it?

If I was to establish a serological diagnostic service for dengue diagnosis, “PanBio Dengue Duo IgM and IgG Capture ELISA” would be my choice for the ideal serological diagnostic assay, using the article “Comparison of five serological diagnostic assays for the detection of IgM and IgG antibodies to dengue virus” by Sekaran SD, Ew CL, Subramaniam G as reference for data.

As we all know, dengue virus is transmitted via mosquito namely the Aedes aegypti and Aedes albopictus. These mosquitoes can be widely found in the tropical and subtropical of the worlds. Currently, there are 4 known serotypes of the dengue virus. In primary dengue infection, there are mild to high fever, headache, bone/joint pain, muscle pain and skin rash associated with it. The IgM antibodies produced in the human body 5 days after the symptoms appear and may persist up to 60 days. The IgG antibodies produced around 14 days after symptoms appear and may persist for life. As for secondary dengue infection, there are high fever with cases of hemorrhagic events, hepatomegaly, thrombocytopenia and circulatory failure. The IgG antibodies rise rapidly 1-2 days after the onset of symptoms and may induce production of IgM antibodies after 20 days since the onset of infection.

*This figure shows the immune response of the human body (which includes NS1 antigens) at different time stages of the infection. []

The statements below are the reasons as to why PanBio Dengue Duo IgM and IgG Capture ELISA is the ideal choice for serological diagnostic assay in serological diagnostic service.

The PanBio ELISA IgM and IgG shows a high value of sensitivity and specificity, yielding it the second highest efficiency among the 5 kits tested in the article, after Anti-Dengue Virus IgM Detection PA Kit 96 [Pentax Corporation]. Furthermore, PanBio ELISA IgM has a high specificity (almost 100%) to other non-specific febrile infection which includes measles, herpes and rubella. With these high values of sensitivity, specificity and efficiency, we can minimize the occurrence of false result which could psychology and financially burden the patients where further test is needed to be carried out.

As stated in the article, the PanBio ELISA tests are able to detect both IgM and IgG simultaneously in a single well. This feature helps to save cost and time as it negates the need of serial dilutions as required by the hemagglutination inhibition (HI) assay. Although the PanBio ELISA test takes a few more hours longer to be carried out compared to the other kits, its advantage to diagnose a large number of samples compensate for these few hours setback. Early diagnosis of dengue infection especially secondary dengue infection is important as through early diagnosis, appropriate measure can be taken such as replenishment of loss body fluids can prevent the occurrence of dengue shock syndrome.

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